Effect of the Tocotrienol-Rich Fraction (TRF) on the Healthspan of Caenorhabditis elegans

Vitamin E is an established antioxidant. However, the effect of vitamin on healthspan, which deteriorates during ageing, has not been determined because most related studies have emphasized its effects lifespan. Therefore, purpose this study was to determine palm tocotrienol-rich fraction (TRF) lifespan, locomotion and thermotolerance Caenorhabditis elegans, share many common gene sequences with humans. The nematodes were treated different concentrations TRF (0 - 200 μg/mL), number surviving at each concentration (N=30, duplicate) counted daily under a light microscope optimal dose treatment. divided into 3 groups, namely; control, Tween-80 (vehicle) TRF-treated. Locomotion day 4 12 treatment in adult nematodes. ImageJ used for analysis, based nematode survivals after exposure 37 °C. TRF-treated C. elegans had significantly longer lifespans compared controls (P = 0.003). group (50 μg/mL) longest mean lifespan (23.5 days), controls, (18.5 days; 0.002). similar between all groups. In assay, survival TRF-treatment higher (P= 0.046). Interestingly, Tween- 80-treated showed results as controls. findings indicate that prolongs increases but does improve worms they age.